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KMID : 0357419990290030145
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Korean journal of Virology
1999 Volume.29 No. 3 p.145 ~ p.154
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Construction of a Novel Recombinant Baculovirus Producing Polyhedra with Bacillus thuringiensis Cry1Ac Crystal Protein
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여연호/1Yeon Ho Je
2Byung Rae Jin/1Jin Hee Chan/2Seok Kwon Kang
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Abstract
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We have now constructed a novel recombinant baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) producing polyhedra with Bacillus thuringiensis (Bt) CryIAc crystal protein. The recombinant polyhedra produced by the recombinant baculovirus, Btrus, in insect cells was characterized. The recombinant baculovirus has two independent transcription units in opposite orientations with two promoters, p10 or polyhedrin gene promoter each initiating transcription of either native polyhedrin or fusion protein with polyhedrin and Bt CrylAc crystal protein. Surprisingly, this recombinant baculovirus stably produced recombinant polyhedra which were nearly similar to those of wild-type AcNPV. The immunogold staining experiment showed that the recombinant polyhedra were assembled with polyhedrin and Bt Cry1Ac crystal protein, and contained virus particles. Insecticidal toxicity of recombinant polyhedra of Btrus to the faU webworm, Hyphantria cunea, was strikingly improved in comparison with the wild-type AcNPV.
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